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A fluorescent cationic dye ex/em spectra 502/525 460/650 and 475/590 nm when bound to dsDNA bound to ssDNA or RNA and under acidic conditions respectively ratio of emission at 590 to emission at 525 nm can be used to quantify the increase in acidic vesicular organelles observed during cellular autophagy
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A phospholipid has been used in the formation of PERLs that reduce cellular cholesterol levels in human PMBCs and decrease the secretion of HIV-1 particles in PBMCs infected with HIV-1LAI has been used as a component of lipid monolayers and small unilamellar vesicles to study the effect of phospholipid composition on membrane fluidity
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101314 of human Olig2 Host Rabbit Species Reactivity ( ) Human Mouse Rat Applications WB MW 32 kDa
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Immunogen Synthetic peptide from the N-terminal region of the a3 subunit of the rat GABAA receptor Host Rabbit Species Reactivity ( ) Mouse Rat Applications IHC WB MW 51 kDa
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A metabolite of all-trans retinoic acid formed through metabolism of all-trans retinoic acid by CYP26A1 B1 and C1 binds to RARs with a lower affinity than other all-trans retinoic acid metabolites (IC50s 606 298 and 892 nM for RARa RARB and RARY respectively in a radioligand binding assay) transactivates RARs with a lower efficacy than other metabolites (EC50s 791 64 and 94 nM for RARa RARB and RARY respectively) but induces transcription of a reporter plasmid equipotently inhibits cell growth halts the cell cycle in the G1 phase and induces differentiation of NB4 acute promyelocytic leukemia cells (EC50 79 8 nM)
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A chiral building block has been used in the synthesis of a chiral synthetic antibiotic intermediate as well as in the stereoselective synthesis of NK receptor antagonists
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Features a biotin group and propanoic acid separated by a disulfide linkage allows biotinylation of targets through either a chemical or enzymatic acylation reaction disulfide bond permits removal of the biotin group with reducing agents
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A muscarinic receptor antagonist (Kis 1.62 27.5 and 2.63 nM for M1 M2 and M3 receptors respectively) inhibits carbachol-induced contraction of isolated human iris sphincter circular ciliary muscle and longitudinal ciliary muscle (Kbs 7.9 15.8 and 12.5 nM respectively)
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An ectoparasiticide and antipruritic agent blocks mouse TRPV4 channel expressed in HEK293 cells in a calcium-dependent manner (IC50s 223.5 and 15.5 M in buffer containing 0 and 2 mM calcium respectively) inhibits scratching behavior in mice induced by the TRPV4 agonist GSK1016790A topical application inhibits scratching behavior in mice induced by histamine serotonin and the PAR2 agonist SLIGRL-NH2 (0.025 g of a 10 ointment)
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Antigen recombinant mouse ANGPTL3 Host Rat Cross Reactivity ( ) Mouse ANGPTL3 ( ) mouse ANGPTL4 and human ANGPTL3 Application(s) ELISA and WB ANGPTL3 regulates angiogenesis and also directly regulates lipid glucose and energy metabolism
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A selectve antagonst of DP1 (K 1 6 nM) blocks PGD2nduced plasma exudaton n the conjunctva (ED50 0 099 mg/kg) and suppresses antgennduced allergc responses n gunea pgs
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Cayman s Thromboxane B2 (TXB2) Express ELSA Kt Monoclonal s a compettve assay that can be used for quantfcaton of TXB2 n serum urne tssue culture supernatants and other sample matrces The assay has a range from 15 62000 pg/ml and a senstvty (80 B/B0) of approxmately 45 pg/ml
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